Caloric Restriction in Saccharomyces cerevisiae as a Model for Intracellular Changes During Aging

3 Introduction 4 Figure 1: Survival Curves 5 Figure 2: Cytochrome c Oxidase Structure 8 Figure 3: Caloric Restriction Pathways 12 Methods and Materials 13 Results 20 Figure 4: Subunit Va and Vb Isoform Switching 21 and Quantification Figure 5: Subunit II Quantification 22 Figure 6: Subunit I, II, and Va Alignments 24 Figure 7: Subunit II Phosphorylation 26 Figure 8: Phosphatase/Phosphatase Inhibitor Effect on 27 Cco/NO Activity Figure 9: [ATP]/[ADP] Effects on Cco/NO Activity 29 Figure 10: Glucose Concentrations During Growth 31 Figure 11: NO Production During Growth 32 Figure 12: ATP/ADP Extraction Method Comparison 34 Figure 13: [ATP], [ADP] During Growth 36 Figure 14: ATP:ADP During Growth 37 Discussion 38